Biochemistry and molecular biology

Module Clinical biochemistry and clinical molecular biology

The aim of the course is to make the student understand the role of genetic tests in the patient's clinical path and to provide an updated view of the molecular biology methods that are applied in the clinical field. At the end of the course the student will acquire the knowledge of the application principles of techniques for detecting genetic alterations.

The course takes place through lecture with slide projection and practical exercises; in addition, there will be activities to be carried out independently or in groups. Interactive teaching is foreseen with participation in active working groups and quizzes, through the use of web platforms and Open Source software. 
Written and oral exam. Written examination with multiple choice questions on all the topics covered during the course.
Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus. Learning assessment may also be carried out on line, should the conditions require it.

Clinical Molecular Biology tests.

Clinical molecular diagnostics: biological material.

Spectrophotometric techniques for nucleic acid and protein analysis.

Recombinant biotechnologies for clinical diagnosis, genome and gene expression analysis.

Enzymatic tools of genetic engineering, new generation sequencing platforms, immunochemical techniques.

1) Biologia Molecolare. F. Amaldi, P. Benedetti, G. Pesole, P. Plevani. CEA edizioni.

2) Medicina di laboratorio. Logica e patologia clinica. I. Antonozzi, E. Gulletta. Piccin.

3) Le analisi personalizzate nella medicina di laboratorio. L. Spandrio, B. Milanesi. Piccin.